A Modified Orcinol Reaction for RNA Determination

A modified orcinol reaction for RNA determination.

A MONG the various colorimetric methods for RNA estimation, the orcinol reaction of Mejbaum as modified by Schneider (1) remains the most sensitive and commonly employed technic for purine-bound ribose quantitation. Later attempts that were made to estimate pyrimidine nucleotides and nucleosides (2, 3) have also been based on the color reaction of ribose with orcinol and ferric chloride, which ...

a modified orcinol reaction for rna determination

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Estimation of monosaccharides by the orcinol-sulphuric acid reaction.

application and construction of carbon paste modified electrodes developed for determination of metal ions in some real samples

ساخت الکترودهاِی اصلاح شده ِیکِی از چالشهاِی همِیشگِی در دانش شیمِی بوِیژه شیمِی تجزیه مِی باشد ،که با در نظر گرفتن سادگِی ساخت، کاربردی بودن و ارزان بودن روش مِی توان به باارزش بودن چنِین سنسورهاِی پِی برد.آنچه که در ادامه آورده شده به ساخت و کاربرد الکترودهاِی اصلاح شده با استفاده از نانو ذرات در اندازه گِیرِی ولتامترِی آهن وکادمِیم اشاره دارد. کار اول اختصاص دارد به ساخت الکترود خمِیر کربن اصلاح شده با لِیگاند داِ...

A role for hydrophobicity in a Diels–Alder reaction catalyzed by pyridyl-modified RNA

New classes of RNA enzymes or ribozymes have been obtained by in vitro evolution and selection of RNA molecules. Incorporation of modified nucleotides into the RNA sequence has been proposed to enhance function. DA22 is a modified RNA containing 5-(4-pyridylmethyl) carboxamide uridines, which has been selected for its ability to promote a Diels-Alder cycloaddition reaction. Here, we show that D...

A modified method for the quantitative determination of the thymol turbidity reaction of serum.

It was observed by Maclagan that turbidity is produced in a barbital buffer saturated with thymol when specimens of serum from patients with diseases of the liver are added (1, 2). The degree of turbidity apparently varied with the degree of hepatic parenchymal damage. An analysis of the precipitate demonstrated that it contained globulin, phospholipid, cholesterol, and thymol. The mechanism of...